Skip to main content
. 2019 Jun 18;101(2):445–456. doi: 10.1093/biolre/ioz108

Figure 6.

Figure 6.

SMAD-dependent signaling pathway is required for the BMP6-induced stimulation of CTGF in SVOG cells. (A) SVOG cells were transfected for 24 h with 25 nM of control siRNA (siCtrl) or 25 nM of SMAD4 siRNA (siSMAD4), followed by treatment with vehicle control or 30 ng/ml of BMP6 for 3 h. The mRNA levels of SMAD4 and CTGF were examined using RT-qPCR. (B) SVOG cells were transfected for 24 h with 25 nM of control siRNA (siCtrl) or 25 nM of SMAD4 siRNA (siSMAD4) followed by treatment with vehicle control or 30 ng/ml of BMP6 for 6 h. The protein levels of CTGF and SMAD4 were examined using western blotting. (C) SVOG cells were transfected for 24 h with 25 nM of control siRNA (siCtrl) or 25 nM of SMAD4 siRNA (siSMAD4), followed by treatment with vehicle control or 30 ng/ml of BMP6 for 3 h. The mRNA levels of SMAD4 and CYR61 were examined using RT-qPCR. (D) SVOG cells were transfected for 24 h with 25 nM of control siRNA (siCtrl) or 25 nM of SMAD4 siRNA (siSMAD4) followed by treatment with vehicle control or 30 ng/ml of BMP6 for 6 h. The protein levels of CYR61 and SMAD4 were examined using western blotting. The results are expressed as the mean ± SEM of at least three independent experiments. Values marked by different letters are significantly different (P < 0.05).