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Detection of gaseous nitric oxide in plants through the Griess assay. (a) Reaction mechanism which shows that NO is first oxidized to nitrite and reacts with sulfanilamide to give a diazonium salt. The diazonium salt then reacts with N -(1-naphthyl) ethylenediamine (NED) and forms a magenta coloured azo compound showing absorbance at a maximum of 540 nm. (b) Set-up for measurement of NO using the Gas Phase Griess reagent.
