Fig. 6.

Schematic view of the experimental set-up of MIMS- and RIMS-based NO measurement. (a) In MIMS, a cell suspension in an 8–10 ml reaction chamber is circulated over a thin Teflon membrane by a magnetic stirrer. The dissolved gases diffuse through the membrane and pass through the ionization chamber of the mass spectrometer. (b) In RIMS, the sample can be a leaf of a small plant or root tissue cells which fit into the leaf cuvette. The produced gases pass through the capillary tube before entering the ionization chamber of the mass spectrometer. A throttle valve connected to a capillary tube regulates the flow of gases to the ionization chamber.