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. 2019 Apr 2;28(17):2811–2825. doi: 10.1093/hmg/ddz080

Figure 7.

Figure 7

The naturally occurring Parkin p.V224A hyperactive variant rescued mitophagy in several pathogenic variants. (A) Quantification of GFP intensity from GFP signal by FACS in untreated cells expressing WT GFP-Parkin, pathogenic missense variants or p.V224A in cis with WT or pathogenic variants. The GFP intensity for each Parkin missense variant was normalized to that for WT Parkin in each replicate. (B) Quantification of induced mitophagy after 4 h of CCCP treatment in cells expressing WT GFP-Parkin, pathogenic missense variants or p.V224A in cis with WT or pathogenic variants. Mitophagy mediated by each Parkin missense variant was normalized to that of WT Parkin in each replicate. *P<0.05; **P<0.01, in two-way ANOVA with Dunnett’s post hoc test comparing the function of each variant with variant in cis with p.V224A. N = 3–7.