Fig 2. Transgenic expression of wmk causes a female-biased sex ratio.

Each sample point represents the adult offspring produced by a replicate family of ten mothers and two fathers (average offspring number per data point is 90). Bars represent the average sex ratio. Control gene flies have the Wolbachia transgene WD0034. WT is the BSC8622 strain. E = expressing, NE = non-expressing, Act5c has an Act5c-Gal4 gene, CyO has the CyO chromosome. wmk-expressing flies have a significantly female-biased sex ratio against all other genotypes. This experiment has been done four times. Statistics are based on a Kruskal-Wallis one-way ANOVA followed by Dunn’s correction. **p<0.01, *** p<0.001. Orange dots represent wmk, blue dots represent the control gene, and gray dots represent the WT strain.