Table 1. Comparative genomic analysis of male-killing gene candidates.
After applying all criteria in the genomic analysis, seven candidates for male killing were identified. All seven gene candidates are listed with their functional annotation and locus tags from both wMel and the closely related wRec strain. BLASTP results of the homologs are also shown with the percent coverage, E-value, pairwise identity, and number of nucleotides for each strain. For inclusion and exclusion criteria, see S1 Table. WD0626 from wMel is the gene hereafter denoted WO-mediated killing or wmk.
| Annotation | wMel Locus Tag | wRec Locus Tag | Ref-Seq Coverage | E-Value | Pairwise % Identity | wRec | wMel |
|---|---|---|---|---|---|---|---|
| Ankyrin Repeat | WD0550 | wRec0541 | 100% | 0 | 95% | 789 | 990 |
| Transcriptional Regulator | WD0626 | wRec0560 | 100% | 0 | 99% | 912 | 912 |
| Rpn (Recombination-Promoting Nuclease) | WD0627 | wRec0561 | 100% | 0 | 99% | 897 | 897 |
| Hypothetical Protein | WD0628 | wRec0562 | 100% | 0 | 100% | 540 | 540 |
| CifA (CI Component) | WD0631 | wRec0566 | 100% | 0 | 99% | 1425 | 1425 |
| Rpn (Recombination-Promoting Nuclease) | WD0296 | wRec0561 | 81% | 0 | 88% | 897 | 912 |
| Phospholipase D | WD1243 | wRec1232 | 100% | 0 | 99% | 531 | 531 |