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. 2019 Sep 10;14(9):e0222140. doi: 10.1371/journal.pone.0222140

Fig 1. The protocol of HDRA method.

Fig 1

(A) The fresh tumor tissue of CCA patients after surgery. (B) The tumor tissues were minced into small pieces and weight ~10 mg/piece. (C) The small piece of tumor tissues was placed on sponge gel and culture for 4 days in conditioned media without and with designing drug concentrations, respectively (D) After culturing, the tissues were performed MTT assay and calculated %IR in each condition. Conditions including 1 = control, 2 = 1000 μg/ml gemcitabine, 3 = 1500 μg/ml gemcitabine, 4 = 20 μg/ml cisplatin, 5 = 25 μg/ml cisplatin and 6 = 1000 μg/ml gemcitabine + 20 μg/ml cisplatin.