Fig 4. M. leprae induces NETs formation in vitro.
Neutrophils from LL/BL, T2R, and T2Rthal patients were isolated and stimulated or not with M. leprae whole-cell sonicate (MLWCS) for 90 min. (A) Immunostaining of NETs components (MPO, green; histone, red; and DNA, blue). Arrows indicate the presence of NETs. Images are representative of 3 LL/BL, 4 T2R, and 4 T2Rthal. Scale bar: 20 μm. (B) DNA release was measured in culture supernatants by picogreen. Box plots show median, interquartile range, sample minimum, and maximum. Each dot represents a donor. **P<0.01 (Mann Whitney test).