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. 2019 Sep 10;14(9):e0222355. doi: 10.1371/journal.pone.0222355

Table 4. Specificity, sensitivity, positive and negative predictive values of observed child hand cleanliness by E. coli contamination categories.

Hand indicators E. coli level
In hand rinse (MPN per 100 ml)
Number of samples in category Number of dirty a observations Number of clean observations Specificity,
% (95% CI)
Sensitivity,
% (95% CI)
PPV, % (95% CI) NPV, % (95% CI)
Fingernails
<1 333 245 88 26 (22, 32)
1–9 178 145 33 82 (75, 87) 37 (32, 42) 73 (64, 80)
10–99 43 35 8 81 (67, 92) 13 (9, 17) 92 (84, 96)
≥100 27 21 6 78 (58, 91) 8 (5, 12) 94 (87, 98)
All≥1 248 201 47 81 (76, 86) 45 (40, 50) 65 (57, 73)
Finger pads
<1 333 77 256 77 (72, 81)
1–9 178 47 131 26 (20, 34) 38 (29, 47) 66 (61, 71)
10–99 43 14 29 33 (19, 49) 15 (9, 25) 90 (86, 93)
≥100 27 11 16 41 (22, 61) 13 (6, 21) 94 (91, 97)
All≥1 248 72 176 29 (24, 35) 48 (40, 57) 59 (55, 64)
Palms
<1 333 84 249 75 (70, 79)
1–9 178 46 132 26 (20, 33) 35 (27, 44) 65 (60, 70)
10–99 43 14 29 33 (19, 49) 14 (8, 23) 90 (85, 93)
100 27 12 15 44 (26, 65) 13 (7, 21) 94 (91, 97)
All≥1 248 72 176 29 (24, 35) 46 (38, 54) 59 (54, 63)
Overall handsb
<1 333 246 87 26 (22, 31)
1–9 178 145 33 82 (75, 87) 37 (32, 42) 73 (64, 80)
10–99 43 35 8 81 (67, 92) 13 (9, 17) 92 (84, 96)
≥100 27 22 5 82 (62, 94) 8 (5, 12) 95 (88, 98)
All≥1 248 202 46 82 (76, 86) 45 (40, 50) 65 (57, 73)

MPN: Most probable number; CI: Confidence interval; PPV: Positive predictive value; NPV: Negative predictive value.

aDirty defined as containing visible dirt particles or having unclean appearance.

bComposite indicator combining fingernails, finger pads and palms(i.e., any of these hand parts dirty).