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. 2019 Jul 30;142(9):2705–2721. doi: 10.1093/brain/awz209

Figure 5.

Figure 5

DREADD effects are regionally specific within the hippocampus. DREADDs do not affect behavioural measures of anxiety, showing dorsal hippocampal specificity. (A) Representative images of open field analysis performed on the SOR habituation trial. Left images show ambulation tracking with boundary for anxiety analysis. Right images show heat maps of ambulation tracking depicting the cumulative time (s) the animal spent in each location. (B and C) Thigmotaxis analysis. While epileptic animals display anxiogenic behaviour, DREADDs do not affect performance [B: F(3,41) = 7.011, P = 0.0006; C: F(1.30, 12.96) = 1.502, P = 0.251]. (D) Representative images of open field analysis for control animals in a novel open field arena with their respective CNO doses. (E) Thigmotaxis analysis [F(4,56) = 1.769, P = 0.148]. Statistical comparisons were done with either an ordinary one-way ANOVA (B and E) or a repeated measures one-way ANOVA with Geisser-Greenhouse correction (C), all with Tukey’s multiple comparisons correction. Data are represented as mean ± SEM. Each circle represents an animal (number of animals indicated in parentheses). nsP > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001. WT = wild-type.