Figure 3.
Transcriptional profiles of Lgr5hi and Bmi1+ cells are profoundly influenced by diet. Lgr5EGFP,creERT2 or Bmi1creERT2, Rosa26tom mice were randomized to different diets at weaning, the Bmi1 group given a single injection of TAM at 3 months, then all mice sacrificed 2 days later and Lgr5hi or Bmi1creERT2 marked cells isolated by FACS from purified intestinal crypts (Supplementary Figure S1). RNAs from these cells were then isolated and used for RNAseq. n = 3 mice per group (data in Supplementary Table S2, diets defined in Supplementary Table S1). An additional group was RNAseq analysis for Lgr5hi cells from Lgr5EGFP,creERT2, Vdrloxp/loxp mice fed AIN76A control diet from weaning and given a single injection of Tamoxifen to genetically inactivate the Vdr gene in Lgr5hi cells 3 days before sacrifice at 3 months of age. PCA of RNAs from mice fed different diets are shown: (A) Lgr5hi cells; (B) Bmi1creERT2 marked cells. For comparison, in (B), PCA for Lgr5hi cells is shown to be distinct from Bmi1creERT2 marked cells for each of AIN76A, NWD1 and NWD2 diets.