Figure 7.
Validation studies of genes altered by BDE-47 in NPCs. A, Concentration-dependent expression alterations in proposed mRNA targets of PBDEs using qRT-PCR (n = 6 independent experiments). Expression values (−ΔΔCT) were normalized to housekeeping genes (GAPDH and ACTB) and adjusted by the respective vehicle control. Significant differences (p < .05) due to exposure as compared with the vehicle control are indicated by double-crosses (‡, ANOVA) or asterisks (t test). B, Expression changes in proposed PBDE targets between microarray and qRT-PCR experiments at 1 or 10 µM (R2 = 0.8).