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. Author manuscript; available in PMC: 2019 Sep 10.
Published in final edited form as: Regul Pept. 2002 Nov 15;109(1-3):115–125. doi: 10.1016/s0167-0115(02)00194-5

Fig. 2.

Fig. 2.

Confocal analysis. Confocal images show the expression of PACAP (panel A) and PAC1 (panel C) in HCT8 cells. Anti-PACAP or anti-PAC1 rabbit polyclonal Abs, followed by Alexa-488-conjugated goat anti-rabbit IgG, were used. In panels B and D, cells were incubated with rabbit IgG, followed by Alexa-488-conjugated goat anti-rabbit IgG. PAC1-transfected NIH-3T3 cells (panel E) and untransfected NIH-3T3 (panel F) were used as PAC1 positive and negative controls, respectively.