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VEGF mRNA. (Top) VEGF mRNA was determined by Northern blot 8 h after no additions, and the addition of 100 nM PACAP-27 and 100 nM VIP to NCI-H1299 cells. (Bottom) Equal amounts of RNA were loaded onto the gels based on ethidium bromide staining of the 28S and 18S rRNA. This experiment is representative of three others.
