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. 2019 Aug 21;10:1920. doi: 10.3389/fimmu.2019.01920

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Copyright © 2019 Lackner, Weber, Baur, Haffner-Luntzer, Eiseler, Fois, Gebhard, Relja, Marzi, Pfeifer, Halvachizadeh, Lipiski, Cesarovic, Pape, Kalbitz and TREAT Research Group.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Mitochondrial respiration, cellular reactive oxygen species (ROS) and Caspase 3/7 activity of human cardiomyocytes treated for 6 h with 100 ng/ml Midkine. Oxygen consumption rate (OCR) of human cardiomyocytes (Control, Midkine) during Seahorse MitoStress Assay (OCR in pmol/min/E630 vs. time in min) (A). Basal respiration of human cardiomyocytes (OCR in pmol/min/E630) (B). Spare respiratory capacity of human cardiomyocytes (OCR in pmol/min/E630) (C). Amount of reactive oxygen species (mean fluorescence intensity, MFI) (D). Caspase 3/7 activity (Luminescence in counts/sec) (E). Results are presented as mean ± SEM. For all experiments n = 6. Data were analyzed by two-tailed, unpaired students t-test. Results are significant *p < 0.05.