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. 2019 Aug 27;10:1891. doi: 10.3389/fmicb.2019.01891

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Copyright © 2019 Leong, Schmid, Toi, Wang, Irudayaswamy, Goh, Bosshard, Glatz and Dawson.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Table comparison of commensal yeast species detection by culture-dependent and culture independent methods in four subjects and (B) PCR detection using species-specific primers of gDNA isolated from the parallel side of the skin of the nose from which cultures were swab-derived. gDNA isolated from reference strain cultures representative of common isolates of Malassezia (CBS 9369, CBS 7222, CBS 7966, CBS 7877, CBS 7956), Rhodotorula (ATCC 9449), and Candida (ATCC 22019) were used as positive controls. A 100 bp ladder (GeneRuler^TM) was run on the leftmost column of each gel.