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. Author manuscript; available in PMC: 2020 May 16.
Published in final edited form as: Cell Chem Biol. 2019 Feb 28;26(5):662–673.e7. doi: 10.1016/j.chembiol.2019.01.012

FIGURE 3.

FIGURE 3.

Inhibition of CXCL12-induced recruitment of β-arrestin-2 to WT CXCR4 expressed on mammalian cells. Values represent the mean from at least two independent experiments, and error bars refer to the standard error of the mean (SEM). A. The kinetic traces for each drug treatment are shown. B. Dose-response curves generated from the kinetic data shown in Fig. 3A at time = 20 minutes. While the antagonists displayed various potencies in preventing β-arrestin-2 signaling, they displayed similar levels of efficacy, with all being able to completely inhibit signaling at concentration of 10 μM. Luminescence is proportional to association of the labeled proteins. All graphs normalized to untreated controls for each individual trial. See also Table 1 for quantified results.