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. Author manuscript; available in PMC: 2019 Sep 10.
Published in final edited form as: Mol Biochem Parasitol. 2010 Nov 24;176(1):25–36. doi: 10.1016/j.molbiopara.2010.11.011

Fig. 4.

Fig. 4

Subcellular localisation of TbEIF4E1 through 4 in T. brucei procyclic forms. (A) Subcellular localisation of the four eIF4E homologues in wild type T. brucei procyclic cells (WT 427) by indirect immunofluorescence. This was performed using the antibodies directed to the various homologues followed by incubation with the Alexa Fluor 488 conjugated secondary antibody. (B) The localisation of TbEIF4E1 through 4 was also confirmed through the expression of eYFP fusion proteins in transfected T. brucei procyclic cells examined under the confocal microscope.