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. Author manuscript; available in PMC: 2019 Sep 10.
Published in final edited form as: Mol Biochem Parasitol. 2010 Nov 24;176(1):25–36. doi: 10.1016/j.molbiopara.2010.11.011

Fig. 7.

Fig. 7

Metabolic labelling of procyclic cells from select RNAi curves. (A) Total protein synthesis was estimated after RNAi for TbEIF4E3 (E3) and the double constructs TbEIF4E1/2 (E1/E2) and TbEIF4E1/4 (E1/E4). At selected time points (3 and 4 days for the first two samples and 3, 4 and 8 days for TbEIF4E1/4), aliquots of the cells were incubated in the presence of 35S methionine followed by TCA precipitation and quantitation of the incorporated radioactivity. To estimate the efficiency of translation in the RNAi treated cells in comparison with the minus tetracycline controls, the normalized incorporation values after two hours of incubation were used to calculate the ratios shown in the figure. (B) For a qualitative analysis of the proteins being synthesized, aliquots of 2x106 cells derived from an independent labeling experiment (4 days after tetracycline addition) were ran on an SDS-PAGE gel followed by autoradiography.