KEY RESOURCES TABLE
| Reagent or resource | Source | Identifier |
|---|---|---|
| Antibodies | ||
| mouse anti-polyglutamine (1C2) | Millipore | Cat#MAB1574; RRID: AB_94263 |
| rabbit anti-Ubiquitin | Cell Signaling Technology | Cat#3933S; RRID: AB_2180538 |
| rabbit anti-LC3B | MBL International Corporation | Cat#PM036; RRID: AB_2274121 |
| rabbit anti-Hsp40 | Cell Signaling Technology | Cat#4868; RRID: AB_2094249 |
| mouse anti-Hsp70 | Abcam | Cat#ab5439; RRID: AB_304888 |
| mouse anti-Hsp90β (K3705) | Enzo Life Sciences | Cat#ADI-SPA-842-F |
| mouse anti-ATXN3 (1H9) | Millipore | Cat#MAB5360; RRID: AB_2129339 |
| mouse anti-p62 | Enzo Life Sciences | Cat#ADI-SPA-902-D;RRID: AB_2039203 |
| mouse anti-GAPDH | Millipore | Cat#MAB374; RRID: AB_2107445 |
| rabbit anti–α-Tubulin | Cell Signaling Technology | Cat#S2144; RRID: AB_10638206 |
| rabbit anti-BECLIN1 | Abcam | Cat#ab207612; RRID: AB_2692326 |
| rabbit anti-OCT4 | Cell Signaling Technology | Cat#2750; RRID: AB_823583 |
| rabbit anti-SOX2 | Cell Signaling Technology | Cat#3579; RRID: AB_2195767 |
| rabbit anti-NANOG | Cell Signaling Technology | Cat#4903; RRID: AB_10559205 |
| mouse anti-vimentin | Invitrogen | Cat#MA3-745; RRID: AB_326285 |
| mouse anti-NESTIN | Cell Signaling Technology | Cat#33475; RRID: AB_2799037 |
| rabbit anti-MAP2 | Cell Signaling Technology | Cat#4542; RRID: AB_10693782 |
| goatanti-PSMD11 | Santa Cruz Biotechnology | Cat#sc-79275; RRID: AB_2172965 |
| Experimental Models: Cell Lines | ||
| Human: UM4-6 hESC line (NIH approval number NIHhESC-12-0147) | MStem Cell Laboratory of Gary Smith, University of Michigan | WT-hESC |
| Human: UM134-1 hESC line (NIH approval number NIHhESC-14-0286) | MStem Cell Laboratory of Gary Smith, University of Michigan | SCA3-hESC |
| Oligonucleotides | ||
| anti-ATXN3 antisense oligonucleotide: 5’-GCATCTTTTCATACTGGC-3’ |
Ionis Pharmaceuticals | ASO |
| Scrambled control antisense oligonucleotide: 5’-GTTTTC AA AT AC ACCTTC-3’ |
Ionis Pharmaceuticals | ASO-Ctrl |
| Primers for LIN28: 5’-AGCCATATGGTAGCCTCATGTCCGC-3’ 5’-TCAATTCTGTGCCTCCGGGAGCAGGGTAGG-3’ |
This paper | N/A |
| Primers for OCT4: 5’-CCCCAGGGCCCCATTTTGGTACC-3’ 5’-ACCTCAGTTTGAATGCATGGGAGAGC-3’ |
This paper | N/A |
| Primers for SOX2: 5’-TTCACATGTCCCAGCACTACCAGA-3’ 5’-TCACATGTGTGAGAGGGGCAGTGTGC-3’ |
This paper | N/A |
| Primers for NANOG: 5’-TTGGGACTGGTGGAAGAACC-3’ 5’-GATTTGTGGGCCTGAAGAAA-3’ |
This paper | N/A |
| Primers for AFP: 5’-AAACTATTGGCCTGTGGCGA-3’ 5’-GGCCAACACCAGGGTTTACT-3’ |
This paper | N/A |
| Primers for TUBB3: 5’-ATGCGGGAGATCGTGCACAT-3’ 5’-CCCTGAGCGGACACTGT-3’ |
This paper | N/A |
| Primers for BRACHYURY: 5’-ACCCAGTTCATAGCGGTGAC-3’ 5’-GGATTGGGAGTACCCAGGTT-3’ |
This paper | N/A |
| Primers for ATXN3: 5’-GGAACAATGCGTCGGTTG-3’ 5’-GCCCTAACTTTAGACATGTTAC-3’ |
This paper | N/A |
| Primers for NESTIN: 5’-GGGAAGAGGTGATGGAACCA-3’ 5’-AAGCCCTGAACCCTCTTTGC-3’ |
This paper | N/A |
| Primers for MAP2: 5’-TTGGTGCCGAGTGAGAAGA-3’ 5’-GTCTGGCAGTGGTTGGTTAA-3’ |
This paper | N/A |
| Primers for HPRT 5’-GGACCTCTCGAAGTGTTGGATA-3’ 5’-ACGTGATTCAAATCCCTGAAGT-3’ |
This paper | N/A |
| Primers for ATXN3 Gene Fragmentation Analysis: 5’- ACAGCAGCAAAAGCAGCAA-3’ 5’- CCAAGTGCTCCTGAACTGGT −3’ |
This paper | N/A |
| Software and Algorithms | ||
| Image J Analysis Software | Abramoff et al. 2004 | https://imagej.nih.gov/ij/? |