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. Author manuscript; available in PMC: 2020 Sep 15.
Published in final edited form as: J Immunol. 2019 Aug 7;203(6):1650–1664. doi: 10.4049/jimmunol.1801236

Figure 10. D reading frame usage based on the GRAVY index.

Figure 10.

For each sequence in the experiment data, we determined if it was using the D segment reading frame that encoded for the lowest GRAVY index in the reference germline. For each subject and set of DN B cells and IgD−CD27+ sequences, the frequency of usage of the minimum GRAVY frame for each D allele was calculated as the number of positive events over the total number of calls of the D allele. (A) For DN and IgD−CD27+ memory B cells, mean usages for HC and MS patients were retrieved for each allele and these values were used to calculate differences HC minus MS. The figure shows the distribution of the difference in mean usage of the minimum GRAVY frame for all the alleles (n = 32), in the DN and IgD−CD27+ memory B cell compartments. (B) A similar comparison method was used to explore differences between DN and IgD−CD27+ memory B cells of HC (top) and MS patients (bottom). The figure shows the distribution of the difference (DN minus IgD−CD27+) in mean usage of the minimum GRAVY frame for all the alleles in HC (top) and MS (bottom).