KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CTb | List Biological, | goat polyclonal, Cat.# 703, lot# 115037, RRID: AB_10013220 |
| dsRed | Clontech, | rabbit polyclonal, Cat.# 632496, RRID: AB_10015246 |
| mCherry | Life Sciences, | rat monoclonal, Cat.# M11217, RRID: AB_2536611 |
| GFP | Invitrogen, | chicken polyclonal, Cat.# A10262, RRID: AB_2534023 |
| TH | Millipore, | mouse monoclonal, Cat.# 657010, RRID: AB_212601 |
| Mouse, goat, rabbit or rat | Invitrogen, | Alexa fluorophore −488, −594 or −647, Cat.# A21203, A-21202, A-31571, A-11058, A-11055, A-21447, A-21207, A-21206, A-31573, A-21208, A-21209 |
| Bacterial and Virus Strains | ||
| AAV8.hSyn.DIO.hM3Dq.mCherry | University of North Carolina Vector Core | |
| AAV9.CBA.Flex.rev.ChR2(H134R).mCherry | University of Pennsylvania Vector Core | Addgene #18916 |
| AAV8.DIO.mCherry | University of North Carolina Vector Core | Cat.# 4981 |
| AAV1.hSyn.Flex.GCaMP6s | University of Pennsylvania Vector Core | Addgene #100845 |
| AAV9.CAG.ChR2(H134R).mCherry | University of Pennsylvania Vector Core | Addgene #20928 |
| AAV10.EF1a.mCherry.DIO.dtA | P. Fuller, M. Lazerus, Beth Israel Deaconess Medical Center | |
| AAV8.hSyn.Cre.mCherry | University of North Carolina Vector Core | |
| AAV8.hSyn.mCherry | University of North Carolina Vector Core | |
| AAV8.DIO.Syp.YFP | Virovek | |
| AAV8.CAG.Flex.RG | Stanford Medicine, Gene Vector and Virus Core | GVVC-AAV-59 |
| AAV8.EF1α.Flex.TVA.mCherry | University of North Carolina Vector Core | |
| SAD.ΔG.EnvA.GFP | Salk Institute for Biological Studies, Viral Vector Core | Cat.# 32635 |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| Experimental Models: Organisms/Strains | ||
| Oligonucleotides | ||
| Recombinant DNA | ||
| Software and Algorithms | ||
| ImageJ | [65] | https://imagej.nih.gov/ij/ |
| LabChart | National Instruments | |
| Prism 7 software | GraphPad (San Diego, CA, USA) | https://www.graphpad.com |
| Microsoft Excel | Microsoft | |
| Adobe Illustrator | Adobe | https://www.adobe.com |
| Doric customized scripts | Doric Lenses | http://doriclenses.com |
| Flir, ResearchIR | Flir | https://www.flir.com |
| Adobe Photoshop | Adobe | https://www.adobe.com |
| Matlab | Mathworks | https://matworks.com |
| Matlab custom scripts | This paper | Available on request |
| Python custom scripts | [62] | NA |
| VLC media player | VideoLAN | http://www.videolan.org |
| OlyVIA software | Olympus | https://www.olympus-lifescience.com |
| VS-ASW software | Olympus | https://www.olympus-lifescience.com |
| Arduino Software, Sketch | Arduino cc | https://www.arduino.cc |
| Clampfit 10 | Molecular Devices | https://www.moleculardevices.com |
| Mini Analysis 6 software | Synaptosoft | http://www.synaptosoft.com/MiniAnalysis/ |
| Other | ||
| Other | ||