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. Author manuscript; available in PMC: 2020 Sep 9.
Published in final edited form as: Dev Cell. 2019 Jul 3;50(5):610–626.e4. doi: 10.1016/j.devcel.2019.06.008

Figure 2. Phosphorylation of C-I and C-II promotes Ci activity in vivo.

Figure 2.

(A-D’”) Control (A-A”) wing disc or wing discs expressing UAS-Myc-Ci-PKA_WT (B-B”), UAS-Myc-Ci-PKA_AA (C-C”), or UAS-Myc-Ci-PKA_DD (D-D”) under the control of C765 Gal4 drive from larvae grown at 25 °C were immunostained to the expression of Ci (red) and Ptc (green). The corresponding adult wings were shown in A’”-D’”. (E-G’”) Wing discs expressing UAS-Myc-Ci-PKA_WT (E-E”‘), UAS-Myc-Ci-PKA_AA (F-F”‘), or UAS-Myc-Ci-PKA_DD (G-G”‘) under the control of C765 Gal4 drive from larvae grown at 18 °C were immunostained to the expression of Ci (red) and Ptc (green). Large magnification views of the posterior compartment of the wing discs are shown in E’”-G’”.

Scale bars are 50 μM for wing discs and 500 μM for adult wings. Also see Figure. S3.