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. Author manuscript; available in PMC: 2020 Sep 9.
Published in final edited form as: Dev Cell. 2019 Jul 3;50(5):610–626.e4. doi: 10.1016/j.devcel.2019.06.008

Figure 7. ULK3/STK36 mediates Gli2 phosphorylation and activation.

Figure 7.

(A) Family tree of Fu-related kinases.

(B-C) Western blot analysis of Myc-Gli2 phosphorylation in NIH3T3 cells transfected with the indicated Gli2 and ULK3 constructs.

(D, F, H) Relative Gli1 mRNA levels measured by RT-qPCR in clonal NIH3T3 cells transduced with lentiviruses expressing Cas9 and the indicated sgRNA with or without the indicated rescue constructs and Shh-N.

(E, G, I) Western blot analysis of Myc-Gli2 phosphorylation in clonal NIH3T3 cells transduced with lentiviruses expressing Cas9 and the indicated sgRNA with or without the indicated rescue constructs and Shh-N treatment.

(J) Western blot analysis of Gli2 phosphorylation in in vitro cultured SmoM2-driven medulloblastoma cells transduced with lentiviruses expressing Myc-Gli2WT or Myc-Gli2AA and treated with the indicated siRNA.

(K) Relative growth of in vitro cultured SmoM2-driven medulloblastoma cells treated with the indicated siRNAs and with or without lentiviral transduction of the indicated Gli2 constructs. Data in D, F, H, K are mean ± SEM from two independent experiments. *p < 0.05, **p < 0.01, and ***p < 0.001 (Student’s t test). Also see Figures S4–S7.