KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Mouse Anti-Flag antibody | Millipore Sigma | Clone ID: M2; Cat#1804; RRID: AB_439685 |
| Mouse Anti-HA (F-7) antibody | Santa Cruz Biotechnology | Cat#sc-7392; RRID: AB_627809 |
| Mouse Anti-Myc antibody | Santa Cruz Biotechnology | Clone ID: 9E10; Cat#sc-40; RRID: AB_627268 |
| Rabbit Anti-Myc antibody | Abcam | Cat#ab9106; RRID: AB_307014 |
| Rat Anti-Ci antibody | DSHB | Cat#2A1; RRID: AB_2109711 |
| Mouse Anti-Fu antibody | DSHB | Cat#22F10; RRID: AB_528258 |
| Goat Anti-PKC ζ antibody | Santa Cruz Biotechnology | Clone ID: c-20; Cat#sc-216; RRID: AB_2300359 |
| Mouse Anti-α-tubulin antibody | Millipore Sigma | Clone ID: DM1A; Cat#T9026; RRID: AB_477593 |
| Mouse Anti-GFP antibody | Takara Biol | Cat#632380; RRID: AB_10013427 |
| Mouse Anti-acetylated Tubulin antibody | Millipore Sigma | Clone ID:6-11b1; Cat#T7451; RRID: AB_609894 |
| Goat Anti-Gli2 antibody | R&D Systems | Cat#AF3635; RRID: AB_2111902 |
| Goat Anti-Gli3 antibody | R&D Systems | Cat#AF3690; RRID: AB_2232499 |
| Bacterial and Virus Strains | ||
| NEB5-Alpha Competent E. Coli | New England Biolabs | Cat#C29871 |
| BL21 Competent E.Coli | New England Biolabs | Cat#C2530H |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Leptomycin B | Millipore Sigma | Cat#L2913 |
| Acti-Stain 670 phalloidin | Cytoskeleton | Cat#PHDN1-A |
| Bluo-Gal | Thermo Fisher Scientific | Cat#15519028 |
| L-Glutathione Reduced | Millipore Sigma | Cat#G4251 |
| SAG | Millipore Sigma | Cat#566660 |
| DAPI | Millipore Sigma | Cat#D9542 |
| ATP [γ −32P] | Perkin Elmer | Cat#BLU002A |
| Insulin | Millipore Sigma | Cat#I6634 |
| Fetal Bovine Serum | Millipore Sigma | Cat#F4135 |
| Fetal Bovine Serum | Millipore Sigma | Cat#F0926 |
| Calf Bovine Serum Iron Fortified | ATCC | Cat#ATCC30-2030 |
| 3× Flag Peptide | Millipore Sigma | Cat#F4799 |
| HA Synthetic Peptide | Thermo Fisher Scientific | Cat#26184 |
| Casein Kinase I | New England Biolabs | Cat#P6030 |
| Recombinant Human Sonic Hedgehog Protein (Shh-N) | R&D Systems | Cat#8908-HSH/CF |
| Lambda Protein Phosphatase | New England Biolabs | Cat#P0753 |
| Critical Commercial Assays | ||
| Dual Luciferase Reporter Assay System | Promega | Cat#E1910 |
| MEGAscript T7 High Yield Transcription Kit | Thermo Fisher Scientific | Cat#AM1334 |
| pIMAGO-biotin Phosphoprotein Detection Kit | Millipore Sigma | Cat#67221 |
| Anti-Flag M2 Affinity Gel | Millipore Sigma | Cat#A2220 |
| Pierce Anti-HA Argarose | Thermo Fisher Scientific | Cat#26181 |
| Glutathione Sepharose 4B | GE Healthcare | Cat#17075601 |
| SimplyBlue SafeStain | Thermo Fisher Scientific | Cat#LC6060 |
| RNeasy Plus Mini Kit | Qiagen | Cat#74134 |
| High Capacity cDNA Reverse Transcription Kit | Applied Biosystems | Cat#4368814 |
| Fast SYBR Green Master Mix | Applied Biosystems | Cat#4385612 |
| CellTiter-Glo Luminescent Cell Viability Assay | Promega | Cat#G7572 |
| Experimental Models: Cell Lines | ||
| Mouse: NIH/3T3 | ATCC | Cat#ATCC CRL-1658 |
| Human: HEK293T/17 | ATCC | Cat#ATCC CRL-11268 |
| D. melanogaster. S2 | Thermo Fisher Scientific | Cat#R69007 |
| D. melanogaster. S2R+ | DGRC | Cat#150 |
| D. melanogaster. Clone 8 | DGRC | Cat#151 |
| S. frugiperda: Sf9 | Thermo Fisher Scientific | Cat#11496015 |
| Experimental Models: Organisms/Strains | ||
| Mouse: CAGGS-CreER; R26-SmoM2 | Mao et al., 2006 | N/A |
| Mouse: Gli3TAP Knock-In | Zhang et al., 2019 | N/A |
| D. melanogaster. UAS-Cr-PKA-WT | Han et al., 2015 | N/A |
| D. melanogaster. UAS-C-PKA-AA | This Paper | N/A |
| D. melanogaster. UAS-C-PKA-DD | This Paper | N/A |
| D. melanogaster. UAS-CiWT | This Paper | N/A |
| D. melanogaster. UAS-CiAA | This Paper | N/A |
| D. melanogaster. UAS-CiDD | This Paper | N/A |
|
D. melanogaster. C765-Gla4 y1 w*; P{GawB}C-765 |
Bloomington Drosophila Stock Center | FBti0002765 |
| Oligonucleotides | ||
| Please see Supplemental Table 2 for oligo/primer sequences | N/A | N/A |
| Recombinant DNA | ||
| Please see Supplemental Table 3 for the list of plasmids used in this study | N/A | N/A |
| Software and Algorithms | ||
| GraphPad Prism | GraphPad Software | https://www.graphpad.com/scientific-software/prism/ |
| ImageJ | NIH | https://imagej.nih.gov/ij/ |