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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Mol Microbiol. 2019 Jun 11;112(3):785–799. doi: 10.1111/mmi.14316

Fig. 4. Two rounds of cell division.

Fig. 4.

Four still frames (one per row) from the time-lapse movie (Movie S1) showing two consecutive rounds of H. volcanii (H98 + pIDJL40-FtsZ1) cell division. The left-hand images show the phase-contrast micrographs which were used to automatically generate cell outline (shown in yellow). The second column of images shows the FtsZ1 fluorescence with the cell outline superposed (yellow). The third column of images shows the predicted division planes (magenta lines) based on the cell shape. The far right column of images are the overlay of the FtsZ1 fluorescence and the predicted division plane. The first round of cell division (top two rows) correspond to movie frames 17 and 19, while the second round of cell division (bottom two rows) correspond to movie frames 36 and 43. Successive frames are separated by 10 minutes.