Skip to main content
. Author manuscript; available in PMC: 2020 Sep 9.
Published in final edited form as: Dev Cell. 2019 Jul 18;50(5):658–671.e7. doi: 10.1016/j.devcel.2019.06.011

Figure 5. DUX4 blocks interferon-γ-mediated up-regulation of MHC Class I-dependent antigen presentation.

Figure 5.

(A) Mean expression of genes encoding the illustrated components of the interferon-g signaling pathway, where the mean was computed over all DUX4− and DUX4+ cancers for each type. Error bars, standard deviations estimated by bootstrapping. TPM, transcripts per million.

(B) As (A), but for the illustrated datasets.

(C) As (A), but illustrating gene expression during preimplantation embryonic development.

(D-I) Immunoblots probing MHC Class I, DUX4, and GAPDH protein following treatment of the indicated cell lines, each of which was engineered to contain a doxycycline-inducible DUX4 expression construct, with interferon-γ (IFN-γ) and/or doxycycline (Dox) to induce DUX4. a-MHC I, pan-MHC Class I probe.

(J-K) Levels of MHC Class I on the cell surface following treatment of the indicated cell lines with interferon-γ (IFN-γ) and/or doxycycline (Dox) to induce DUX4. Cell surface levels of MHC Class I were probed with a pan-MHC Class I antibody.

See also Figure S5.