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. Author manuscript; available in PMC: 2020 Sep 10.
Published in final edited form as: Lab Chip. 2019 Sep 10;19(18):3022–3031. doi: 10.1039/c9lc00354a

Figure 1. Establishment of the progressive NAFLD model.

Figure 1.

(A) Cartoon demonstration of lipid accumulation in hepatocytes during NAFLD progression, which can be achieved in vitro by an increasing supply of free fatty acid (FFA) concentration. (B) Visual demonstration of gradient generation in the microfluidic device. Experimental gradient was created by supplying one inlet with regular media (0 mM linoleic acid, LA) and the other with 2 mM LA. The inlets were connected to two syringes controlled by a single pump. These media were mixed in the gradient generator and were fed to each channel in the cell culture chambers labelled 1–5. Artificial food colouring was added to the media in the two syringes in this image to demonstrate formation of gradients.