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. Author manuscript; available in PMC: 2020 Sep 9.
Published in final edited form as: Curr Biol. 2019 Aug 8;29(17):2790–2800.e4. doi: 10.1016/j.cub.2019.07.012

Figure 1. tai-expressing wings penetrate thoracic cuticle.

Figure 1.

(A-E) Adults expressing UAS-tai from MS1096, nubbin (nub), or rotund (rn) Gal4 lines at 25°C or 18°C. Penetrance is indicated (n=# wings). (F-G) 18hr APF nub>GFP and nub>tai,GFP pupal cryosections. Magnified top (F’,G’) and side (F”,G”) views of GFP+ wing tissue (dotted line denotes cuticle). (H) nub>tai,tub>Gal80ts shifts between 25°C (blue) and 29°C (red). E0=0hr embryo, L1/2/3=1st, 2nd or 3rd instar; WPP=white prepupa. Invasion frequencies (%) are indicated (‘lethal’ = no adults). See also Figure S1.