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. Author manuscript; available in PMC: 2020 Sep 9.
Published in final edited form as: Curr Biol. 2019 Aug 8;29(17):2790–2800.e4. doi: 10.1016/j.cub.2019.07.012

Figure 7. Tai induced apoptosis within the wing epithelium.

Figure 7.

Paired adult wings and larval expression of cleaved Dcp-1 (red) and GFP (green) in (A) ptc>GFP, (B) ptc>GFP,caspc04227/+, (C) ptc>GFP,nec10/+, (D) ptc>Toll10b,GFP, (E) ptc>tai,GFP, (F-F’) ptc>tai,GFP,caspc04227/+, (G) ptc>tai,GFP,nec10/+, (H) ptc>tai,GFP,caspIR, and (G) ptc>tai,GFP,spzIR. Arrow in (F) indicates leg disc. Yellow dashes outline ptc-Gal4 domain. (J) Total Dcp-1+ cells/disc and (K) fraction within ptc>GFP domain. Black bars mark averages. Significance values: *p<0.05; **p<0.01; ***p<0.001.(L) qPCR of tai, rpr, hid, puc, and spz6 in ptc>+ (white) and ptc>tai (grey) wing discs relative to rp49. Errors bars: SD of three biological replicates. (M-Q)hid-GFP (red) and RFP (green) in (M) ptc>RFP, (N) ptc>UAS-Toll10b,RFP, (O) ptc>tai,RFP, (P) ptc>tai,caspIR,RFP, and (Q) ptc>tai,spzIR,RFP wing discs. (R-V) Anti-Dorsal (red), GFP (green), and DAPI (blue) in (R) ptc>GFP control, (S) ptc>UAS-Toll10b,GFP, (T-U) ptc>tai,GFP, (V) and ptc>tai,spzIR discs. Bottom panels (R-V) are magnified views of dashed boxes. Dotted lines indicate anterior:posterior (AP) boundary. See also Figures S6–7.