Table 1.

Borrelia burgdorferi proteins identified by LC‐MS/MS analysis of spots on two‐dimensional polyacrylamide gels such as those shown in Fig. 4.

Spot ID	Protein description	Annotation	MASCOT Score	Isoelectric point (pI)	Theoretical molecular mass (Daltons)
U‐1	Putative Outer membrane protein	BBA03	97.4	5.27	19,222
U‐2	OmpH	BB0796	381.4	7.88	20,513
U‐3	Outer surface protein P66	BB0603	2286.3	6.04	68,172
U‐4	Pfs, 5′‐methylthioadenosine/S‐adenosylhomocysteine nucleosidase	BB0375	348.9	6.98	26,586
U‐5	OspD	BBJ09	130.3	5.37	28,434
U‐6	FlaB, flagellin	BB0147	2390.6	5.53	35,765
U‐7	50S ribosomal protein L25/Ctc, general stress protein	BB0786	270.5	6.75	20,450
D‐1	SodA, superoxide dismutase	BB0153	160.7	6.05	23,523
D‐2	LA‐7, lipoprotein	BB0365	128.6	5.53	21,866
D‐3	Putative transcription factor/CarD like	BB0355	108.9	6.12	19,020
D‐4	OMS28, porin	BBA74	1065.9	6.05	27,948
D‐5	SpoIIIJ‐associated protein	BB0443	107.5	9.46	28,267

Each identified protein that appeared to be differentially expressed as given an identification code (Spot ID) with prefix U or D, depending upon whether enhanced expression of BpuR resulted in the apparent protein level going up or down, respectively. Note that only protein D‐1 (SodA) was investigated further, and the apparent effects of BpuR on the other proteins remain to be clarified. For example, spot U‐6 was identified as the flagellin, FlaB, the major component of the borrelial flagella. Both strains appeared to be equally motile, raising the possibility that BpuR may have influenced a posttranslational modification of FlaB that caused a shift in motility on two‐dimensional polyacrylamide gels.