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. 2019 Aug;7(16):378. doi: 10.21037/atm.2019.07.20

Figure 3.

Figure 3

Overexpression MiRNA-188 aggravates apoptosis in CM treated HK-2 cells. (A) miRNA-188 expression in HK-2 cells transfected with miRNA-188 mimics (miR-188 mimics) or negative control miRNA (mimics-NC) determined by RT-qPCR; (B) expression of Bcl-2, Bcl-xl, Bax, and Caspase 3 determined by western blot analysis in CM-treated HK-2 cells transfected with miRNA-188 mimics (CI-AKI miR-188 mimics) or negative control miRNA (CI-AKI NC). (C) apoptosis levels (green cells) measured by TUNEL assay in normal HK-2 cells, CM-treated HK-2 cells, and CM treated HK-2 cells transfected with miRNA-188 mimics or negative control miRNA (*P<0.05, **P<0.01, *** P<0.001, respectively); the measurement data were expressed as mean ± standard deviation; data differences between the two groups were analyzed by independent-sample t test.