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. 2019 Aug;7(16):378. doi: 10.21037/atm.2019.07.20

Figure 4.

Figure 4

MiRNA-188 induces apoptosis by targeting SRSF7 in cells. (A) The miRNA-188 binding sites in the 3' UTR of the human SRSF7 transcript by target gene analysis, and plasmid with and without the miRNA-188 binding site in the 3' UTR sequence of SRSF7; the WT and MUT sequences were AAGGGAU and CCAAAGG respectively; (B) SRSF7 expression in HK-2 cells transfected with miRNA-188 mimics (miR-188 mimics) or negative control miRNA (mimics-NC) determined by RT-qPCR; (C) targeting of SRSF7 by miRNA-188 determined by the dual-luciferase reporter assay; (D) SRSF7 expression in HK-2 cells treated with siRNA against SRSF7 and negative control siRNA; (E) cell viability rate determined by MTS assay in CM-treated HK-2 cell and CM-treated HK-2 cells transfected with CM-treated HK-2 cells (*P<0.05, **P<0.01, ***P<0.001, respectively); the measurement data were expressed as mean ± standard deviation; data differences between the two groups were analyzed by independent-sample t test.