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. 2019 Sep 10;9:12937. doi: 10.1038/s41598-019-49419-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of GM-CSF and G-CSF on elastase secretion. Neutrophils were incubated with either GM-CSF (A) or G-CSF (B) at the concentration indicated for 50 min in the absence (white columns) or presence (grey columns) of 1 μM fMLP. fMLP was used as positive control. Elastase levels were measured in the supernatants. Results are shown as mean ± SEM of five experiments. ANOVA (with Tukey’s post hoc test) and Student t-test. In both panels, **P < 0.01: +fMLP controls vs. -fMLP controls; *P < 0.05 and **P < 0.01 as compared with untreated and –fMLP controls or untreated and +fMLP controls.