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. 2019 Sep 10;9:12962. doi: 10.1038/s41598-019-49344-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression of exhaustion markers in the spleen of dogs naturally infected with L. infantum. Ex vivo analysis by qPCR of the mRNA levels in the spleen of dogs classified according to the clinical score. Gene expression values were normalized to those of the constitutive genes HPRT and GAPDH. ANOVA: (A) p < 0.022. (*) Mann-Whitney test: (B) p < 0.036.