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. 2019 Sep 10;9:12973. doi: 10.1038/s41598-019-49480-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Silencing PDK1 significantly elevates the cytoplasmic ROS level. (A) HCT116 cells with or without the transduction of a PDK1 shRNA were cultured in normal condition. Flow cytometry showed ROS levels had no obvious difference regardless of PDK1 status. (B) As indicated in (A), cells were cultured in matrix detachment. ROS levels were significantly enhanced by silencing PDK1, compared with the controls. (C) Histogram indicated the corresponding comparison of ROS levels presented in normal and anoikis culture conditions in (A,B). Data expressed as mean ± S.D., **** represents P < 0.0001.