Fig. 1. Cunningham Panel values of all patients included in our data collection.

The healthy control group has been previously published⁴. These samples were all taken at the time of our study in plastic tubes with a serum separator gel (i.e., Gold Top tubes). Group distributions were compared using Kruskal–Wallis test. Post hoc analysis of medians between groups was made using Mann–Whitney test. There was no difference in CaMKII activation or dopamine receptor D2 antibody between any of the groups. Healthy controls had higher dopamine receptor D2 antibody (p = 0.23), Lyso GM1 antibody (p < 0.01), and β-tubulin antibody (p < 0.01) than the confirmed PANS group. Removing participants who had been treated with IVIG did not change results. Adults had higher Lyso-GM1 antibodies than children, but all other analytes were independent of age