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. 2019 Sep 10;9:13008. doi: 10.1038/s41598-019-49477-3

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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RPL28 expression levels influence proliferation of colorectal cancer cells. (a,b) Efficient knock-down of RPL28 protein expression in HCT116 and HT-29 exposed to specific siRNAs (siRPL28) relative to cells transfected with scrambled non-targeting (NT) negative control (siCTR). A representative cropped Western blot is shown for each cell line and a quantification of expression levels based on densitometry of blots from three independent experiments are shown. Full-length blots are shown in Additional file 1: Supplementary Fig. 2. (c–f) The knock-down of RPL28 impairs cell proliferation. Viable cells were monitored up to 96 hours after siRNA transfection in (c) HCT116 and (e) HT-29 (n = 3 in duplicate). Cell proliferation was measured by MTS assays 96 hours post-transfection in (d) HCT116 and (f) HT-29 (n ≥ 3 in triplicate). Data are presented as mean ± S.E.M. *P < 0.05; ***P < 0.001.