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. 2019 Sep 10;10(9):657. doi: 10.1038/s41419-019-1895-4

Fig. 5. Degraded NETs of co-infecting bacteria as a source for growth factors promote growth of A.pp.

Fig. 5

a S. suis was used in a co-infection assay as a DNase source in the absence or presence of porcine neutrophils. Furthermore, the DNase-deficient mutant S. suisΔssnA ΔendAsuis was used as control. The survival factor was calculated based on the CFU at 0 and 3 h. Data shown are mean ± SD (n = 6, one-way ANOVA −PMN P = 0.0006, +PMN P < 0.0001, followed by Dunnet’s multiple comparison test to RPMI and #P < 0.01, one-tailed paired Student’s t-test). b Whole blood of four piglets from the same sow were incubated with A.pp serotype 2 or 7 as single infection or co-infection with S. suis wildtype (wt) or the respective DNase mutant (ΔssnA) for 2 h. The CFU/ml was determined by plating and the survival factor (SF) calculated. In blood of three piglets A.pp was efficiently killed with a SF < 0.05. Data presented from the survival of A.pp in the blood of one piglet