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. 2019 Sep 10;9:12999. doi: 10.1038/s41598-019-49082-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Enhanced rate of endocytosis in Wnt-treated HEK293 cells. Representative capacitance recordings of Wnt3a and Wnt5a-treated HEK293 cells in the cell-attached mode showing successive downward steps corresponding to permanent endocytosis. Imaginary and real part of admittance, corresponding to changes in membrane conductance (ΔG upper trace) and capacitance (ΔC lower trace) for control, Wnt3a treated and Wnt5a treated HEK293 cells. (B) Number of permanent and transient events per patch in control (n = 82), Wnt3a (n = 37) and Wnt5a-treated (n = 41) HEK293 cells. Each patch was measured on a separate cell and the first 500 s were used for the evaluation.