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. 2019 Sep 10;10(9):659. doi: 10.1038/s41419-019-1879-4

Fig. 2. Human recombinant tryptase affects human melanoma cell phenotype and expansion.

Fig. 2

Human melanoma cells (MEL526) were incubated for 48 hours with 50 nM human recombinant tryptase. a Microscopy analysis revealing gross morphological alterations in melanoma cell treated with tryptase. b Assessment of cellular size showing that tryptase causes a reduction in melanoma cell size. c, d Quantification of viable cells using Trypan Blue exclusion (c) and CellTiter-Blue® viability probe (d). e Dead cells were quantified using Trypan Blue positivity. Data in (b–e) are given as mean values ± SEM; *p ≤ 0.05, **p ≤ 0.01, ****p ≤ 0.0001