Fig. 3. Tryptase causes decreased proliferation of melanoma cells.

Melanoma cells (MEL526) were incubated for 48 hours with 50 nM human tryptase. a After incubation, melanoma cells were stained with Annexin V/Draq7 to monitor apoptosis (Annexin V+/Draq7−) and necrosis (Annexin V+/Draq7+). Note that tryptase does not induce apoptosis/necrosis in melanoma cells. b Quantification of the Annexin V/Draq7 staining. c Tryptase-treated melanoma cells (MEL526) were assessed for proliferation through EdU staining. Note the decreased EdU staining after treatment of melanoma cells with tryptase. d–g Quantification of EdU staining of MEL526 (d), MM466 (e), MM253 (f), and A375 (g) melanoma cells. Data in (b, d–g) are given as mean values ± SEM; **p ≤ 0.01, ***p ≤ 0.001; ns, not significant