Table 2.
Experimental vaccination schemes.
| Exp | Chicken type | Antigen | Group | Dose | Age at priming | Prime-boost interval | Blood collection | ||
|---|---|---|---|---|---|---|---|---|---|
| Name | Size | post-prime | post-boost | ||||||
| Exp 1 | Broiler | rH5-E. coli | B-25/2 | 8 | 25 μg | 1 week | 2 weeks | 2 weeks | 2 weeks |
| B-25/4 | 8 | 25 μg | 1 week | 4 weeks | 2 weeks /4 weeks | 1 week/2 weeks | |||
| Broiler | rH5-BEVS | B-25_ref/2 | 7 | 25 μg | 1 week | 2 weeks | 2 weeks | 2 weeks | |
| B-25_ref/4 | 8 | 25 μg | 1 week | 4 weeks | 2 weeks /4 weeks | 1 week/2 weeks | |||
| Broiler | None | B-controls | 15 | n/a | n/a | n/a | 2 weeks 2 weeks /4 weeks | 2 weeks 1 week/2 weeks | |
| Exp 2 | Layer | rH5-E. coli | L-25/4 | 10 | 25 μg | 3 weeks | 4 weeks | 4 weeks | 1 week/2 weeks |
| L-15/4 | 10 | 15 μg | 3 weeks | 4 weeks | 4 weeks | 1 week/2 weeks | |||
| L-10/4 | 10 | 10 μg | 3 weeks | 4 weeks | 4 weeks | 1 week/2 weeks | |||
| L-5/4 | 10 | 5 μg | 3 weeks | 4 weeks | 4 weeks | 1 week/2 weeks | |||
| L-25/6 | 10 | 25 μg | 3 weeks | 6 weeks | 6 weeks | 1 week/2 weeks | |||
| L-15/6 | 10 | 15 μg | 3 weeks | 6 weeks | 6 weeks | 1 week/2 weeks | |||
| L-10/6 | 10 | 10 μg | 3 weeks | 6 weeks | 6 weeks | 1 week/2 weeks | |||
| L-5/6 | 10 | 5 μg | 3 weeks | 6 weeks | 6 weeks | 1 week/2 weeks | |||
| Layer | None | L-controls | 15 | n/a | n/a | n/a | 4 weeks 6 weeks | 1 week/2 weeks | |
The data for the tested (rH5-E. coli) and reference (rH5-BEVS) antigens are summarized in Table 1. The test vaccine groups were denoted according to chicken type (broiler, B or layer, L), the test or reference antigen dose in μg (25 or 25_ref, 15, 10, or 5), and the time interval between doses in weeks (2, 4, or 6). The control groups for vaccination studies in broiler chickens in Exp 1 and layer chickens in Exp 2 were denoted B-controls and L-controls, respectively. Details on the vaccination scheme and sample collection are provided in the Materials and Methods section.