Figure 3.
R. tanguticum nanoparticles inhibition of HSV-1 replication. (A) HEp-2 cells in 96-well plates were treated with serial dilution of R. tanguticum nanoparticles for 72 h and the viability were measured by the MTT assay. (B) Inhibitory effects of R. tanguticum nanoparticles on HSV-1 infection. HEp-2 cells infected with HSV-1 were treated with different concentrations of R. tanguticum nanoparticles and were subjected to the plaque reduction assay. (C) Time-of-addition assay. HEp-2 cells were infected with HSV-1 and then treated with R. tanguticum nanoparticles (350 µg/ml) at indicated intervals. The progeny virus yields were determined by plaque assay. Values are represented as the mean ± standard deviation of three individual experiments. “VC” is the abbreviation of the “virus control group”.*p < 0.05; **p < 0.01; ***p < 0.001; n.s., not significant.