Figure 4.

WSSV activates reductive carboxylation at the WSSV genome replication stage (12 hpi) and suppresses it at the late stage of WSSV replication (24 hpi). At 12 h after challenge with WSSV or PBS, shrimps were injected with [1-¹³C]glutamine and hemocytes were collected (A) 10 min or (B) 30 min later. At 24 h after challenge with WSSV or PBS, shrimps were also injected with [1-¹³C]glutamine and hemocytes were collected (C) 10 min or (D) 30 min later. Metabolomic analysis of pooled hemocyte samples by LC-ESI-Q-TOF-MS was used to calculate the fold change of each ¹³C metabolite in the WSSV group compared to the corresponding ¹³C metabolite in the PBS group. Each bar represents the mean ± SD. Asterisks indicate statistically significant differences between WSSV group and the corresponding PBS control group (*p < 0.05, **p < 0.01, ***p < 0.001). (E,F) Schematic representation of ¹³C metabolic expression of glutamine metabolism in the reductive direction at (E) 12 and (F) 24 hpi at 10 min after [1-¹³C]glutamine treatment. Changes in the WSSV groups relative to the corresponding PBS controls are color coded as follows: green (significant decrease), yellow (no significant difference), red (significant increase), gray (not measured), and white (non-detectable). Abbreviations are the same as for Figure 1.