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. 2019 Sep 4;10:2102. doi: 10.3389/fimmu.2019.02102

Figure 8.

Figure 8

Ras is a primary regulator of reductive glutamine metabolism at the WSSV genome replication stage. (A) Schematic representation of the Ras and PI3K-Akt-mTOR pathways and the three inhibitors used in this experiment (black boxes). Shrimp were treated with the various inhibitors or vehicle only 2 h before WSSV infection. (B) Real-time PCR analysis shows that the RAS inhibitor Salirasib had a significant effect on the expression of GDH, IDH1, and IDH2 in shrimp hemocytes after WSSV infection. (C) Real-time PCR analysis shows that the PI3K-mTOR inhibitor LY29400 had no significant effect on the expression of IDH1, IDH2, and α-KGDH in shrimp hemocytes after WSSV infection. (D) Real-time PCR analysis shows that the mTORC1/C2 inhibitor Torin 1 had a significant effect on the expression of IDH1, IDH2, and α-KGDH in shrimp pleopods after WSSV infection. Bars represent the mean ± SD. Asterisks indicate statistically significant differences in WSSV-injected shrimp between the inhibitor treated groups and the corresponding vehicle-only control (*p < 0.05, **p < 0.01).