Figure 1.

Target sites for gRNAs targeting Ms1, and the vector used for transformation. (a) The gene structure of Ms1 and its homoeologs is shown. The detail underneath shows partial sequences for Ms1 and its homoeologs, as well as the target sites for the three gRNAs (LTPG1‐1, LTPG1‐2 and LTPG1‐4) targeting exon 1 of Ms1 on chromosome 4BS. Target sites are indicated by coloured bars (grey, green and purple). PAM sites (5′‐NGG‐3′) are indicated by black bars at the ends of the coloured bars. Downward‐pointing coloured arrow heads indicate the position of the canonical cut site, and the predicted specificity of the gRNA based on the number and distribution of gRNA mismatches (red text). (b) T‐DNA (12.8 kb) of the Agrobacterium binary vector used for transformation. LB, left border; RB, right border.