Figure 4.

GYY4137 aggravates mechanical hyperalgesia of TRPA1 KO mice and ameliorates arthritis score of TRPA1 WT animals with K/BxN serum-transfer arthritis. Mechanical pain threshold of the hind paws (expressed in g) of GYY4137-treated (50 mg kg⁻¹ day⁻¹) and vehicle-treated TRPA1 WT and KO mice undergoing K/BxN arthritis on days (A) 3, (B) 5, and (C) 7. Semiquantitative clinical scores of GYY4137- and vehicle-treated TRPA1 WT and KO animals affected by serum-transfer arthritis on days (D) 3, (E) 5, and (F) 7. Time to failure of GYY4137- and vehicle-treated TRPA1 WT and KO mice having K/BxN arthritis in suspension test expressed in seconds on days (G) 3, (H) 5, and (I) 7. Hind paw volume detected by plethysmometry expressed in cm³ in GYY4137- and vehicle-treated TRPA1 WT and KO mice undergoing K/BxN arthritis on days (J) 3, (K) 5, and (L) 7. Whiskers show SEM; lines denote mean values, n = 9–10 arthritic mice per group and n = 6–9 non-arthritic mice per group. *p < 0.05 vs. indicated group. ^# p < 0.05 vs. BxN serum-treated animals.