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Solid-phase binding assays of MsDG-Fc to immobilized recombinant laminins. The conditioned media containing MsDG-Fc or control Fc were prepared as described in Methods. Ninety six-well ELISA plates were coated with recombinant proteins at 20 nM (LM111, LM511, LM521, and LM511-E8) or 60 nM (LM511-E8). After blocking, the wells were incubated with conditioned media containing MsDG-Fc or Fc at room temperature for 1 hour. Bound MsDG-Fc was detected with anti-human IgG Fc antibody.
