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. 2019 Sep 10;9:13010. doi: 10.1038/s41598-019-49510-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myosin-1a remains unchanged with both GLP-2 treatment and IE-IGF-1R KO. Control and IE-IGF-1R KO mice were treated with either vehicle (open bars) or h(GLY²)GLP-2 (closed bars) for 11 d, followed by jejunal mucosal scrapes for either (a) q-RT-PCR or (b) western blot analysis; representative blots are shown, with full length blots in Supplemental Fig. 5 (n = 8–14 for PCR; n = 4–9 for western blot). (c) Jejunal sections were paraffin embedded for immunofluorescent staining; insets show magnified views of boxed areas. Red is myosin-1a, blue is DAPI. Scale bar is 20 µm (n = 4).